Kinases

Gene Target Solutions distributes Kinases including Epicentre Bio (an Illumina Company) T4 Polynucleotide Kinase Enzymes within Australia.   Applications include:

  • Labelling of 5´ termini of DNA and RNA with 32P or 33P for DNA sequencing, blot-hybridization experiments, or transcript mapping using Mung Bean Nuclease, S1 nuclease, or other nucleases.
  • Phosphorylation of oligonucleotide linkers and other DNA or RNA molecules prior to ligation, or for use in ligation amplification reactions with Ampligase® Thermostable DNA Ligase.
  • Preparation of labeled DNA or RNA molecular weight markers for gel electrophoresis and chromatography.

MCLAB Pyruvate Kinase I (pykF)

Pyruvate kinase is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding …

MCLAB T4 Polynucleotide Kinase

T4 Polynucleotide Kinase (PNK) catalyzes the transfer and exchange of the terminal gamma position phosphate of ATP to the 5′-hydroxyl terminus of …