Background Reducers for Improved Fluorescent Stains

Christopher Pratt, PhD October 9, 2020. Like a bright sky obscuring the stars, background fluorescence in microscopy makes specific signals harder to see—only the brightest points shine through. Reducing background improves signal-to-noise and enhances image quality, producing better data. Both the intrinsic fluorescence of samples (autofluorescence) and non-specific binding of …

Tech Tip: Multi-Color Fluorescence Imaging Using Biotium’s Tyramide Amplification Kits

Introduction Biotium’s Tyramide Amplification Kits are powerful tools to achieve immunofluorescence staining of cells or tissues with exceptional sensitivity and signal-to-noise. After binding to primary antibodies or biotinylated primary antibodies, an HRP-conjugated secondary antibody or streptavidin mediates covalent coupling of CF® Dye tyramide to tyrosine-containing proteins in the vicinity of …