Background Reducers for Improved Fluorescent Stains
Christopher Pratt, PhD October 9, 2020. Like a bright sky obscuring the stars, background fluorescence in microscopy makes specific signals harder to see—only the brightest points shine through. Reducing background improves signal-to-noise and enhances image quality, producing better data. Both the intrinsic fluorescence of samples (autofluorescence) and non-specific binding of …