Reverse Transcriptase Selection Guide

Epicentre’s Reverse Transcriptases (see Table below) include MMLV High Performance RT, MonsterScript™ RT, and EpiScript™ RT. MMLV High Performance RT demonstrates significantly greater activity than other commercially available MMLV RT enzymes. MonsterScript RT has the ability to make longer cDNAs and more complete full-length libraries of first-strand cDNA molecules compared to other reverse transcriptases. EpiScript RT, an alternative to SuperScript® II RT, demonstrates more activity than other MMLV RT enzymes.

Enzyme RNase H activity Type cDNA Comparison Optimal Temp Heat Inactivation
MMLV High Performance Reverse Transcriptase Low Recombinant Full length (15 kb) All MMLVs 37-42°C 85°C for 10 min
MonsterScript™ Reverse Transcriptase None Recombinant Full length (15 kb) SuperScript® III 50-60°C 90°C for 5 min
EpiScript™ Reverse Transcriptase None detected Recombinant Full length (15 kb) SuperScript® II 37-42°C 85°C for 5 min