PerfeCTa® DNase I is a high purity, recombinant bovine DNase I preparation that is free of any contaminating RNases or proteases. It provides a simple and rapid solution to eliminate residual genomic DNA from total RNA preparations for expression profiling by reverse transcription quantitative PCR amplification (RT-qPCR) as well as other molecular biology applications. The proprietary Reaction Buffer and Stop Buffer support a simple heat-kill step that permanently inactivates all trace levels of DNase activity. Complete inactivation of DNase I is critical before subsequent cDNA synthesis. Residual, or renatured, DNase will degrade cDNA product and thereby alter apparent expression levels. One unit completely degrades 1μg of dsDNA in 10 minutes at 37º C.
- PerfeCta DNase I (2 U/µL, recombinant DNase I, RNase-free in, 50 mM glycine (pH 7.2), 5 mM calcium acetate, 50%(v/v) glycerol and stabilizers)
- 10X reaction buffer for DNase I
- 10X Stop Buffer
PerfeCTa DNase I is stable for 2 years when stored in a constant temperature freezer at -20°C. The enzyme showed no loss in functional performance after 20 cycles of freezing on dry ice and thawing on ice. However, we recommend that the number of freeze-thaw cycles be kept to a minimum. For onvenience, the 10X Reaction Buffer and 10X Stop Buffer may be stored at 2-8°C to avoid thawing the buffers for each use.
Precautions and Disclaimer
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
Cat# 95150-01K 1000 units (2U/µL)