Mic – Personal qPCR Cycler

 

Mic

The world’s first Magnetic Induction Cycler.
The box is small. The ideas are big.

Meet Mic

Hardware:

speed

Mic uses a patented magnetic induction technology to achieve heating and forced airflow for cooling.  This means faster heating and cooling times.  Back that up with a robust optical system that reads all four channels simultaneously, and running multichannel assays has never been quicker.

MicSpeed

  • Very fast cycling times with 35 cycles possible in less than 25 mins
  • Instruments are available with either 2 (Green/Yellow) or 4 channels
  • Separate high intensity LED excitation source for each channel (uniform excitation output) and a separate emission filter and detector for each channel.

accuracy

 

 

Mic’s spinning aluminum rotor gives unsurpassed temperature uniformity during dynamic and static operations.  All other block-based cyclers only promise static uniformity, which could lead to inaccurate data, as samples aren’t heated and cooled equally.  You might think this level of accuracy requires constant calibration, but Mic’s good to go right out of the box.

accuracy2

You don’t need to calibrate – ever.

  • With an excellent temperature accuracy of +/- 0.25ºC, a first class well-to-well temperature uniformity of +/- 0.05C, combined with superior equilibrium time uniformity (zero seconds for all wells to reach the same temperature); easily classify difficult class IV SNP’s that require melt temperature resolutions of less than 0.1°C.
  • Excellent reproducability between samples and repeatability between runs & instruments ensures the highest level of quantification precision; allowing for the detection of two-fold differences in gene expression levels.
  • Fixed optical path with no moving parts means no optical alignment or calibration, and no need for a ROX reference dye.

size

 

 

Mic takes up less space on the bench than your lab book.  And weighing in at just 2kg, this is the most portable qPCR cycler on the market.  Even four Mic’s stacked together take up less bench space than your current cycler.

mic

  • The magnetic induction technology allows a small instrument footprint to be achieved, by way of its elegant simplicity.
  • The tube format uses 0.1mL strips of four tubes and matching caps, supporting volumes of 10 to 25ul.  A tab on tube 1 ensures correct orientation of tubes into the instrument.
  • High speed centrifugation ensures samples spin down at the start of a run, and during a run will remove bubbles and prevent condensation, eliminating the need for bulky heated lids and external centrifuges.

Connectivity

Multiple Mics can be operated from one workstation so 48 becomes 96, and 96 becomes 192. Bluetooth technology means few cables too.

connectivity3

  • Instruments can communicate via Bluetooth or USB cable.  As many as ten instruments can be run from the one PC allowing for up to 480 samples to be run simultaneously.
  • Individual runs can be collated into a project and analysed together.
  • Installation is plug-and-play.

 

Specs

FRONTBACK
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Physical:  

Height:   130mm (265mm lid open)
Width:    150mm
Length:  150mm
Weight:  2.1kg


Electrical:

AC Input: 100 – 240 VAC, 50/60Hz 4.0


Thermal Performance:

Temperature Accuracy:     +/- 0.25°C
Temperature Uniformity:  +/- 0.05°C
Ramp Rates:                         Heating 4°C/s.    Cooling 3°C/s.


Optical:

Detectors:                     Photodiode per channel
Excitation Sources:      High energy light emitting diode for each channel

Channels:

  • Green:  Ex 465nm Em 510nm
  • Yellow: Ex 540nm Em 570nm
  • Orange: Ex 585nm Em 618nm
  • Red: Ex 635nm Em 675nm

Reaction Vessels:

Samples per Instrument:     47
Reaction Volume Range:      10 – 25ul


Operating Environment:

Temperature:            18 – 35°C
Relative Humidity:    20 – 80%


Software:

It’s not just the hardware that’s more refined. Mic’s software is user-friendly and packed with intelligent features.

Designed to meet MIQE specifications, Mic’s software offers you the most up-to-date qPCR analysis.  With an intuitive interface, setup and analysis is simple, while further control features give you total confidence in your data.

relative quantification

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Mic’s RQ software uses up-to-date mathematical models and well-founded statistical analysis, allowing you to compare gene expression levels for different targets across multiple groups.  All the necessary calculations and statistics are carried out within the software.  Data is reported both numerically and graphically.  With Mic’s superior temperature uniformity, detect difference between samples as little as 0.2 fold.

hrm

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Mic’s optional HRM software characterises DNA samples according to their melt behaviour so you can identify mutations, including difficult Class IV SNPs (in example red samples contain the A base allele, blue T base allele, and purple are heterozygote).  HRM is the perfect tool for applications including determining allele prevalence, screening for loss of heterozygosity, DNA fingerprinting, DNA methylation, species identification and calculating the ratio of somatic acquired mutations.

absol qual

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Using a standard curve, AQ allows you to determine the absolute amount of a genetic target. This 5 point, twofold dilution series produced an efficiency of 98%.  The percentage variation between the given and calculated concentrations was no greater than 5%, allowing for accurate quantification of the unknown sample.

genotyping

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Use differential melt curves from various types of chemistries, including quenched FRET dual hybridization probes, beacon probes or Plexor to characterise a sample’s genotype.  Melt peaks can be inverted to accommodate the different chemistry types.


biomolecular

Created by Bio Molecular Systems

Mic is designed and manufactured by Bio Molecular Systems (BMS), founded by the leading innovators of the former Corbett Life Sciences company,  the original team behind Rotor-Gene 6000, X-Tractor Gene and CAS-1200.

BMS draw on decades of expertise when designing, manufacturing and distributing their laboratory instruments of unparalleled accuracy and functionality.