Extracta™ DNA Prep

Features and Benefits

  • Simple, fast protocol
  • Compatible with laboratory automation
  • DNA is suitable for qPCR, High Resolution Melt Analysis, endpoint PCR and other applications
  • Works with wide range of tissue including hair, buccal swabs and mouse tails
  • Optional 10 minute protocol for high-throughput applications

Extracta Technology

Extracta DNA Prep for PCR is a two-component reagent kit for rapid extraction of PCR-ready genomic DNA from mammalian tissues. Tissues are processed in 35 minutes with little hands-on time. Compared to a conventional proteinase-K process this is significantly faster with fewer handling steps. The genomic DNA is suitable for sensitive downstream PCR applications including end-point PCR, High Resolution Melt Analysis (HRM) and quantitative real-time PCR (qPCR) without requiring any additional clean-up. In addition the extracted DNA may be used in multiplexed PCR applications such as transgene or knock-out analyses. Tissue extractions can be done in tubes, plates or deep-well blocks to allow for adaptation to workflow and automation on liquid-handling workstations.

Compatibility with Quanta Reagents

Extracts are compatible with both endpoint PCR and real-time qPCR reagents including:

  • AccuStart™ PCR SuperMix products for endpoint PCR
  • AccuMelt™ HRM SuperMix for high resolution melting analysis
  • PerfeCTa™ FastMix™ or PerfeCTa SuperMix for SYBR® Green detection
  • PerfeCTa qPCR FastMix or PerfeCTa qPCR SuperMix for TaqMan® assays

 

Performance Data:

 

extractyieldmousetails

Figure 1: Comparison of Extract Yield: Mouse Tails
2 mm tail snips were extracted in 150 L of Extracta DNA Prep for PCR – Tissue or reagents from competitor products according to each manufacturers instructions. 1.5 L of each extract  was used in 20 L PCR reactions of mouse brain-derived neurotropic factor using PerfeCTa SYBR Green FastMix. PCR data was obtained in triplicate and converted to number of copies of target detected using a standard curve generated from purified mouse genomic DNA.

 

 

extractyieldmouseears

Figure 2: Comparison of Extract Yield: Mouse Ears
1 mm diameter mouse ear punches were extracted in 100 L of Extracta DNA Prep for PCR – Tissue or reagents from competitor products according to each manufacturers instructions. 5.0 L of each extract  was used in 20 L PCR reactions of mouse brain-derived neurotropic factor using PerfeCTa SYBR  Green FastMix. PCR data was obtained in triplicate and converted to number of copies of target detected using a standard curve generated from purified mouse genomic DNA.

 

extractaprotocol_speed_comparison

Figure 3: Protocol Speed
Extracta reduces time to results with a rapid, convenient protocol.

 

extractamultiplex_genotyping

Figure 4: Multiplex Genotyping

 

 

Ordering

Extracta™ DNA Prep for PCR – Tissue
Cat# 95091-025 25 mL
Cat# 95091-250 250 mL