RNase A
Applications Removal of RNA from DNA preparations. Removal of unhybridized regions of RNA from DNA-RNA or RNA-RNA hybrids. Ribonuclease A (RNase A) …
FailSafe™ PCR System
Applications Simplified PCR optimization. PCR of difficult or high-GC templates (Fig. 1). Multiplex PCR (Fig. 2). PCR amplifications up to 20 kb …
Ampligase® Thermostable DNA Ligase
Applications Ligation amplification (Ligase Chain Reaction, LCR)1-7 Repeat Expansion Detection (RED).7 Simultaneous mutagenesis of multiple sites.8,9 Other ligation-based detection methods. Ampligase® Thermostable …
RNA 5´ Polyphosphatase
Applications Conversion of 5´-triphosphorylated RNA to 5´-monophosphorylated RNA for use in 5´-RNA ligation-tagging methods using T4 RNA Ligase. Analysis of 5´-end structure …
Terminator™ 5´-Phosphate-Dependent Exonuclease
Applications Characterize the 5´ termini of RNA transcripts. Enrich for mRNA in prokaryotic or eukaryotic total RNA preparations (Fig. 1). Note: For …
Hybridase™ Thermostable RNase H
Applications High-stringency hybrid selection. Diagnostic assays of specific target DNA sequences by isothermal probe amplification.1,2 Transcription-based amplification methods (e.g., NASBA® method).3,4 High-stringency …
RNase III, E. coli
Applications Digestion of long dsRNA to short dsRNA. RNA structure studies.1 RNA processing and maturation studies.2-4 Ribonuclease III (RNase III) from E. …
RNase I, E. coli
Applications Removal of RNA from DNA preparations.1 RNase protection assays to detect single-basepair mismatches in RNA:RNA and RNA:DNA hybrids.1 RNase I degrades …
Rec J Exonuclease
Applications Removal of primers from completed PCR. Degradation of single-stranded linear DNA in dsDNA and plasmid preps Rec J Exonuclease, derived from …
Plasmid-Safe™ ATP-Dependent DNase
Applications Removal of contaminating bacterial chromosomal DNA in large-scale plasmid, cosmid, fosmid, and BAC clone or vector preparations (Fig. 1). Plasmid-Safe™ ATP-Dependent …