FAQ’s

cDNA Synthesis

cDNA synthesis and RNA denaturation step

Contamination of RNA with RNase activity

Difference between qScript cDNA SuperMix and qScript cDNA Synthesis

Primers in qScript™ cDNA SuperMix

Priming strategies for first strand synthesis

Purpose of 25C incubation during cDNA synthesis

qScript™ Flex cDNA Kit vs qScript™ cDNA Synthesis Kit

RNA dynamic range with cDNA Supermix and cDNA synthesis Kit

RNA input for qScript cDNA Supermix

RNA input for qScript cDNA Synthesis Kit

Rnase Inhibitor and cDNA synthesis

scaling up a cDNA synthesis reaction

Stability of MMLV RT in qScript cDNA Supermix and qScript cDNA synthesis

Variability in the efficiency of first strand cDNA synthesis

What is the smallest quantity of RNA detectable by the qScript™ cDNA Synthesis Kit?

PCR

AccuFast and AccuStart Taq DNA Polymerase Activation time

AccuStart PCR SuperMix compatibility with T/A cloning methods

Effect of uracil residues on post PCR hybridization

Improving PCR specificity

Melting temperature (TM) of PCR product

Online registration when contacting technical support

PCR extension temperature

Product shelf life and storage

Reagents protocols, MSDS, PPS

RNA extraction methods

Volume of first strand reaction transferred to PCR

PCR Enzymes

AccuStart™ Taq DNA Polymerase proofreading activity

Activation of AccuStart™ Taq DNA Polymerase

Components of AccuStart™ Taq DNA Polymerase PCR buffer

Difference between Taq DNA Polymerase and AccuStart™ Taq DNA Polymerase

Function of the antibody in AccuStart™ Taq DNA Polymerase

Hot Start PCR

Inactivation of reverse transcriptase (RT)

Optimal Temperature for MMLV reverse transcriptase

Storage and stability of AccuStart™ Taq DNA Polymerase

Storage and stability of M-MLV reverse transcriptase (RT)

Unit definition for AccuStart™ Taq DNA Polymerase

Units of AccuStart™ Taq DNA Polymerase used in PCR

What is Uracil N Glycosylase (UNG)?

qPCR

Absolute versus relative quantification

Advantages of PerfeCTa™ qPCR FastMix™over PerfeCTa™ qPCR SuperMix

Applications for the qPCR FastMix

cDNA input in SYBR and Taqman qPCR

Compatibility of Quanta Biosciences reagents with Roche’s LightCycler 1.5/2.0

Difference between AccuFast™ Taq DNA polymerase and AccuStart™ Taq DNA Polymerase

Expiration date

Fast cycling reaction volume

Improving qPCR specificity

Information to provide when calling technical support

Instrument Compatibility- SYBR Green Detection

Instrument Compatibility-Taqman Probe Detection

Is the PerfeCTa™ qPCR FastMix™ offered with UNG?

Mixing PCR reactions

Multiplexing

One-step or Two-step reaction?

Performance of PerfeCTa™ qPCR FastMix compared with PerfeCTa™ qPCR Supermix

Primer quality/sequence effect on qPCR effeciency

Primer validation/quality

Replicates

Sensitivity of SYBR and Taqman reagents chemistries

Stability at room temperature

Using PerfeCTa™ qPCR SuperMix in Fast mode

What component in the qPCR FastMix allows rapid amplification results?

Probe Detection

Primer and probe concentration in a Taqman reaction

SYBR Detection

SYBR and ABI 7500 instrument

qRT-PCR

Dnase treatment of RNA

How to avoid Dnase treatment?

Inactivation of DNase during Dnase treatment compromises the quality of RNA

Is my RT-PCR product RNA specific?

qRT-PCR kits compatibility with Cell Lysis reagents

qRT-PCR Kits directly from cell lysates

Residual Dnase activity

RNA input in SYBR and Taqman qPCR

Setting up a no-RT (no-reverse transcription) control

Uracil N-glycosylase (UNG) and Reverse transcription