MasterPure™ Yeast RNA Purification Kit
Poster from RNA Society (714k)
Applications
- Purification of RNA from yeast and filamentous fungi suitable for cDNA synthesis and microarray gene expression analysis (Figs. 1 and 2).
The MasterPure™ Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from yeast cell types (including Candida, Saccharomyces, Schizosaccharomyces) and filamentous fungi. The kit uses a rapid salting-out process1 to remove contaminating macromolecules, avoiding toxic organic solvents, bead-beating, and spheroplasting.2
Benefits
- Avoids hot acid phenol.
- Faster than spheroplasting.
- Higher quality RNA than bead-beating.
- No extra enzymes or equipment to purchase.
- Yields 25-50 µg RNA from 1 ml of mid-log S.cerevisiae.
https://www.genetargetsolutions.com.au/wp-content/uploads/2025/07/YEASTRNA.jpg
References
- Miller, S.A. et al., (1988) Nucleic Acids Res. 16, 1215.
- Hoffman, L.M. and Jarvis, B.W. (2004) Epicentre Forum 11(2), 4.
Ordering
| Catalog No. | Size |
|---|
MasterPure™ Yeast RNA Purification Kit
| MPY03100 | 100 Reactions |
Contents: Extraction Reagent for RNA, MPC Protein Precipitation Reagent, TE Buffer (in 100-reaction kit only), Proteinase K, RNase-Free DNase I, RiboGuard™ RNase Inhibitor, 10X DNase Buffer, 2X T & C Lysis Solution.