EvaGreen® dye is a next generation green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), routine solution DNA quantification and capillary gel electrophoresis.
The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR® Green I, making the dye readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling.
The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. EvaGreen dye is nonmutagenic and noncytotoxic by being completely impermeable to cell membranes, unlike SYBR Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer (Ohta, et el. Mutat. Res. 492, 91(2001)).
The unique properties of EvaGreen dye have made it particularly useful in quantitative real-time PCR (qPCR) application. Compared with the widely used SYBR Green I, EvaGreen dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen dye can be used at a much higher dye concentration than SYBR Green I, resulting in more robust signal for both PCR and melt curve analysis.
Non-mutagenic, non-cytotoxic and safe to aquatic life for safe handling and easy disposal down the drain.
Superior for qPCR and isothermal amplification.
Far brighter than SYBR Green 1 for detecting amplification due to novel ‘release on demand’ DNA binding mechanism.
Highly Sensitive. Unrivalled DNA melt curve performance.
Low PCR inhibition permitting use of saturation dye concentration for maximal signal and high-resolution DNA melt analysis.
Nonmutagenic and noncytotoxic
Nonmutagenic and noncytotoxic by standard Ames test; completely impermeable to cell membranes
Serving both as a qPCR dye and a DNA gel stain.
Electrophoretically separated PCR product visualized directly via a UV box without the need for another gel stain.
Compatible with Fast PCR protocol
Minimal interference to PCR makes it possible to significantly shorten the chain extension time
Compatible with multiplex PCR
Lack of dye migration from amplicon to amplicon enabling detection of multiple PCR products by melt curves, when used at the recommended concentration
Unsurpassed Thermal Stability, Hydrolytical Stability and Photostability
Perfectly stable during storage or under PCR condition. No detectable dye decomposition in PCR buffer at 95-100°C for 48 hours; highly stable under either alkaline or acidic condition; withstand repeated freeze-thaw cycles
Spectrally similar to SYBR Green I
Compatible with all major brand qPCR instruments and enzyme systems
Applicable in other applications
Can be used as a general dsDNA binding dye for DNA quantitation in solution, capillary gel electrophoresis and more.
Click here to download brochure
Cat# 31000 EvaGreen Dye, 20X in H20; 5 x 1mL