AccuClear™ Ultra High Sensitivity dsDNA Quantitation Solution utilizes a novel, next-generation DNA binding dye for highly sensitive and accurate quantitation of purified dsDNA samples across a broad range of DNA concentrations. The assay is linear between 0.03 ng and 250 ng of dsDNA per assay in microplate format. Unlike absorbance-based measurements, AccuClear™ dye is highly selective for double-stranded DNA over single stranded DNA or RNA.
The AccuClear™ dsDNA quantitation assay is designed for fluorescence 96-well plate readers equipped with excitation and emission filters for detecting green fluorescence. The unique spectral properties of AccuClear™ dye make it especially well-suited for use with instruments with blue LED excitation sources. Biotium’s AccuLite™ 470 Mini Fluorometer (cat. no. E90001) includes a program for use with the AccuClear™ assay. AccuClear™ also is compatible with handheld fluorometers such as Invitrogen’s Qubit® and Promega’s QuantiFluor®-P, however the standard curve calibration programs for these instruments may not cover the full dynamic range of the AccuClear™ kit standard curve.
The AccuClear™ Ultra High Sensitivity dsDNA Quantitation Solution includes AccuClear™ dye and assay buffer. Biotium also offers the AccuClear™ Ultra High Sensitivity dsDNA Quantitation Kit (catalog no. 31028), which includes dye, buffer, and a set of pre-diluted calf thymus dsDNA standards. For large numbers of samples, Biotium offers the AccuClear™ Ultra High Sensitivity dsDNA Quantitation Kit with 1 DNA Standard (2000 assays, cat. no. 31029) which includes dye, 20X assay buffer, and a 25 ng/uL DNA standard stock solution that can be used to prepare a range of standards.
Standard curve of calf thymus DNA assayed using the AccuClear Ultra High Sensitivity Kit and read on a microplate reader (Ex/Em 460/507).
Inset shows the lower end of the titration.
- Linear range: 0.03-250 ng dsDNA
- Green fluorescence (Ex/Em 460/507 nm)
- Unrivaled sensitivity and dynamic range
- Novel green fluorescent dye is a perfect match for blue LED excitation sources
- Green fluorescence detection compatible with fluorescence microplate reader, handheld fluorometer, or NanoDrop® fluorospectrophotometer.
Please also see related products for information on the AccuBlue™ High Sensitivity dsDNA Quantitation Solution and Kit (0.2-100 ng) and AccuBlue™ Broad Range dsDNA Quantiation Solution and Kit (2-2000 ng), In addition to different linear ranges, these assays have different tolerances to various contaminants compared to AccuClear™ (see product protocols for details).
AccuClear kits are intended for quantitation of purified dsDNA samples.
AccuClear™ technology is covered by pending U.S. and international patents.
Cat # 31027-T – 200 assays (trial size)
Cat # 31027 – 1,000 assays