QuickGene Mini 80 – Semi Automated Nucleic Acid Isolation System

Kurabo’s QuickGene Mini 80 semi automated  Nucleic Acid Isolation System not only rapidly isolates DNA and RNA from a wide range of starting material but also yields high QUALITY DNA and RNA in high QUANTITIES.

QG-Mini80 new

The QuickGene Mini 80 is a very affordable, compact, light weight (3kg), semi automated system used to rapidly isolate high yields of quality DNA and RNA. Centrifugation or vacuum manifolds are NOT required in this isolation process. QuickGene employs the revolutionary, patented, ultra thin (8um) and highly absorptive porous membrane to rapidly isolate high yields of quality DNA and RNA.  The extremely thin membrane reduces the risk of contamination, and allows the processing of samples at mild low pressure conditions without using rigorous centrifuging processes.

DNA isolated by QuickGene has remarkable lengths of 97kbp through its gentle separation process under low pressure conditions. Traditional spin-column isolation methods using a centrifuge have been known to yield shorter DNA fragments than QuickGene, due to the cleavage of DNA molecules. The ultra thin membrane and low pressurizing method reduce damage to the DNA segment.

The QuickGene Mini 80 utilises the same isolation method and the same QuickGene isolation kits as the fully automated QuickGene 810 System.  DNA and RNA can be easily isolated from a variety of samples including whole blood, tissue, cells, plants and viruses.

QuickGene Mini is very compact (280mm W x 220mm D x 180mm H) for minimal bench space requirements, and is easily portable at 3kg in weight.

 

TECHNOLOGY

“QuickGene” products employ a unique porous membrane technology which guarantees high purity and high yield nucleic acid extraction.

Revolutionary Patented Porous Membrane gives High Purity and High Yield!

The revolutionary patented nucleic acid adsorptive medium used with the QuickGene series of instrumentation is an ultra thin porous membrane, only 80μm thick. Because of the high adsorptive and desorptive properties of this ultra thin membrane, nucleic acids can be rapidly and reliably extracted at low pressures without compromising the integrity of the nucleic acid, ensuring high quality nucleic acid extraction. This ultra thin membrane enables nucleic acid isolation in shorter time frames when compared to the glass fibre membranes used by its competitors.

The Proprietary 80μm thick porous membrane effectively absorbs nucleic acids in hydrophobic solvents, effectively separating them from proteins and lipids. Nucleic acids are absorbed onto the membrane due to their hydrophilic properties. Lipids and proteins on the other hand are relatively hydrophobic and tend to seep out of the membrane. This ensures that a stable and reproducible isolation of nucleic acids is accomplished.

QG_technology

 

Easy and Rapid Processing:

The operation is simple. Just set the sample and rotate the pressurizing Rotary Switch on both sides of the QuickGene-Mini80. Centrifugation is not required for the isolation. The operation is simple, and rapid DNA/RNA isolation saves valuable time.

The small, lightweight QuickGene-Mini80 device takes up minimal space on the lab bench and is easy to carry. QuickGene-Mini80 can be operated on the lab bench so, without moving around, the user can perform nucleic acid isolation.

quickgene mini 80 workflow

 

DNA/RNA Extraction from various samples:

QuickGene isolation kits are optimized for the QuickGene system to isolate DNA and RNA in the short time and with high quality.  Appropriate kit selectable depending on samples.

quickgene mini 80 extraction examples

 

SPECIFICATIONS:

Dimensions:
280 (W) x 220 (D) x 180 (H) mm

Weight:
Approx 3kg

Voltage Supply:
AC100 – 240V

Power Supply Frequency:
50/60 Hz

Operating Conditions:
Temperature: 15 – 30 C
Humidity: 30 – 80% RH (non-condensing)

Throughput:
1 to 8 samples per run.

 

QUICKGENE MINI 80 KITS

Kurabo’s wide selection of QuickGene isolation kits are used in conjunction with the QuickGene Mini 80 Nucleic Acid isolation System for optimal isolation of quality DNA and RNA in the shortest amount of time. The kit selection will depend on the starting material and on the application.

DNA Kits for QuickGene 810 / QuickGene Mini 80

NucleicAcid Target Sample Quantity Purified
< Time>
Kit Name Pkg Size Part No
DNA Whole Blood 5 μg/Whole blood 200 μL
< 6 min / 8 samples >
QuickGene DNA whole blood kit S 96 tests DB-S
DNA Tissues (Human; Animal; Plant; Insect; Paraffin-embedded section, etc.), Cultured Cells, Bacteria, Virus, etc. 4 μg/5mg BALB/c Mouse tail
< 13 min / 8 samples >
QuickGene DNA tissue kit S 96 tests DT-S
Plasmid E. coli 12.5 μg/mL cultue pBlueScript II / DH5α
< 6 min / 8 samples >
QuickGene Plasmid kit S II 96 tests PL-S2

 

RNA Kits for QuickGene 810 / QuickGene Mini 80

NucleicAcid Target Sample Quantity Purified
<Time>
Kit Name Pkg Size Part No
RNA Leukocytes 4.5 μg/1 x 107 cells leukocytes
< 20 min / 8 samples >
QuickGene RNA blood cell kit S 96 tests RB-S
RNA Tissues, Virus, etc. 100 μg/30 mg Mouse liver
< 15 min / 8 samples >
QuickGene RNA tissue kit S 96 tests RT-S2
RNA Cultured Cells, Plant, etc. 10 μg/1 x 106 cells HL60 cell
< 17 min / 8 samples >
QuickGene RNA cultured cell kit S 96 tests RC-S
RNA Cultured Cells 90~150 μg/10 cm dish cultured HEK293 cell
< 11 min / 8 samples >
QuickGene RNA cultured cell HC kit S 96 tests RC-S2

COMPARISON WITH QUICKGENE MANUAL SPIN COLUMN METHOD

Target Sample Extract Kit Name Equipment Samples Extraction Time
Whole Blood
DNA
DNA whole blood kit L QuickGene-610L
for 48 samples
12 min / 6 samples
SP kit DNA whole blood Manual (Spin Column Method)
for 96 samples
35 min / 8 samples
Tissues (Human; Animal; Plant; Insect; Paraffin-embedded section, etc.), Cultured Cells, Bacteria, Virus, etc.
DNA
SP kit DNA tissue Manual (Spin Column Method)
for 96 samples
30 min / 8 samples
E. coli
Plasmid
SP kit plasmid II Manual (Spin Column Method)
for 96 samples
40 min / 8 samples
Tissues (Human; Animal; Insect), Virus, etc.
RNA
RNA tissue kit S II QuickGene-Mini80 / 810
for 96 samples
15 min / 8 samples
SP kit RNA tissue Manual (Spin Column Method)
for 96 samples
30 min / 8 samples
Cultured Cells, Plant, etc.
RNA
RNA cultured cell kit S QuickGene-Mini80 / 810
for 96 samples
17 min / 8 samples
SP kit RNA cultured cell Manual (Spin Column Method)
for 96 samples
35 min / 8 samples
Cultured Cells
RNA
RNA cultured cell HC kit S QuickGene-Mini80 / 810
for 96 samples
11 min / 8 samples
SP kit RNA cultured cell HC Manual (Spin Column Method)
for 96 samples
55 min / 8 samples

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