NEW! Swift Amplicon HS Panels

Swift Amplicon HS Panels enable variant calling at and below 1% frequency, with allele sensitivity dependent on the amount of input material available and the depth of sequencing. Using 10 ng of input DNA, this assay consistently detects variants at 0.5% with a recommended sequencing depth of at least 60,000x (1M reads) prior to deduplication with MIDs and 0.25% from 20 ng of input DNA.

Swift Amplicon HS Panels enable variant calling at and below 1% frequency, with allele sensitivity dependent on the amount of input material available and the depth of sequencing.  Using 10 ng of input DNA, this assay consistently detects variants at 0.5% with a recommended sequencing depth of at least 60,000x (1M reads) prior to deduplication with MIDs and 0.25% from 20 ng of input DNA.

Workflow

Supported Applications

  • Oncology – liquid biopsy cfDNA and FFPE tissue
  • Genome/Host Detection – Graft vs. Host Disease and Fetal in Maternal cfDNA
  • Custom targets

The Swift Amplicon HS EGFR Panel consistently detected all expected variants from 10 ng SeraSeqTM ctDNA Reference Material v2 AF0.5% and 20 ng SeraSeq ctDNA Reference Material v2 AF0.25%. Variants were called from an MID-based consensus sequence by LoFreq (Genome Institute of Singapore).

Product Specifications

Resources

Ordering

  •  Cat # HS-51024 Swift Amplicon HS EGFR Panel

The below solutions are included with your product. If you require extra, please advise.

    • Cat # 90196 PEG NaCl Solution (96 rxns)
    • Cat # 90296 Low EDTA TE (96 rxns)