NEW! Swift Amplicon 16S+ITS Panel

The Swift Amplicon 16S+ITS Panel enables highly efficient, sensitive and specific microbial identification by targeting the 16S rRNA (V1-V9) and ITS genes in a single primer pool, along with a simple two-hour workflow.

Comprehensive

  • Targets the entire 16S rRNA gene (V1-V9) and the Fungal ITS1 and ITS2 genes
  • Unique multiplex PCR chemistry generates diverse clusters without PhiX or phased primers, saving up to 25% of sequencing costs

Fast workflow, high-quality data

  • From DNA to Illumina® compatible libraries within 2 hours
  • Faster turnaround time with 2×150 or 2×300 read lengths
  • Sensitive and Specific
  • Accurate quantification, with 100% sensitivity observed with mock microbial communities (ATCC® MSA-1003TM), and 100% specificity validated with microbial isolates.

Supported Applications

  • Detection and characterization of bacterial and fungal structure in complex samples
  • Metagenomics: Bacteria, Archaea, Fungi
  • Environmental: Air, Water, Wastewater
  • Agriculture: Plant microbiome, Soil – ITS for fungal
  • Microbiome: Human – skin, GI tract, stool, vagina, Plant – surface, roots and soil
  • Forensics: Microbiome to establish time of death

Workflow:

The Swift Amplicon 16S+ITS workflow uses a fast, single-tube approach consisting of a 70-minute target enrichment amplification step and a 20-minute adapter ligation step, yielding a 2-hour start-to-finish procedure.

The single-tube workflow includes two steps that generate the multiplex amplicon targets and then add a unique combination of Illumina-compatible indexed adapters, creating up to 96 uniquely-indexed libraries for multiplexing on a single sequencing run.

Panel Specifications:

Performance Data:

FAQ:

What sample types are compatible with the Swift Amplicon 16S+ITS Panel?

  • This panel is compatible with a wide variety of DNA types, including but not limited to DNA from environmental samples, gut microbiomes, and soil samples.

How much DNA is required for the Swift Amplicon 16S+ITS Panel?

  • We recommend 1-50 ng input DNA. It is recommended that input DNA concentration be determined using Qubit, or a similar fluorometric method, as it will accurately represent the double-stranded DNA content of your sample. Sample purity should be determined by spectrophotometry (260/280).

How long does the protocol take to complete?

  • The protocol takes approximately 2 hours from input DNA until finished library ready for quantification.

What targets are covered by this panel?

  • This panel covers the entire 16S rRNA gene (V1-V9), along with the Fungal ITS1 and ITS2.

How do I analyze Swift Amplicon Swift Amplicon 16S+ ITS Panel data?

  • Data from this panel can be analyzed using the following tools:

– Illumina’s 16S Metagenomics BaseSpace
– Illumina’s ITS Metagenomics BaseSpace App
– RDP Tools

Please Note: Other analysis tools are available; however, these have not yet been internally validated.

Ordering:

  • Cat # AL-51648 Swift Amplicon 16S+ITS Plus Panel

The below solutions are included with your product. If you require extra, please advise:

  • Cat # 90196 PEG NaCl Solution (96 rxns)
  • Cat # 90296 Low EDTA TE (96 rxns)

Resources: