The new Accel-Amplicon panels are based on unique molecular biology to provide powerful solutions for detecting and screening clinically relevant mutations.* Swift Biosciences multiplex amplicon panels are comprised of 10’s to 100’s of primer pairs in a single-tube format which are optimized for sequencing on Illumina® platforms. Primer pairs in the panels are designed for compatibility with the short DNA fragments from both FFPE and cell-free circulating DNA samples. A fast and easy single-tube workflow produces the best-in-class performance for on-target percentage and coverage uniformity, enabling variant discovery and confirmation.
|Single-tube assay||Provides easy and consistent processing|
|Ready-to-sequence libraries in 2 hours||Process more samples per day|
|10 ng input DNA required||Process low input samples|
|Average amplicon size of 138-140 bp||Compatible with FFPE and cfDNA|
|Contiguous coverage||Complete coverage of desired regions|
|Uniformity of coverage > 95%||Reads evenly distributed across targets|
|On target > 95%||Reads mapped to intended targets|
Simple, Single-tube Workflow
The Accel-Amplicon workflow uses a fast, single-tube approach consisting of a 90-minute target enrichment amplification step and a 10-minute adapter ligation step, yielding a 2 hour start-to-finish procedure.
High Coverage Uniformity Across Sample Types
10 ng of input DNA from a variety of sample types was used to generate libraries with the Accel-Amplicon 56G Oncology Panel. The coverage uniformity, as the percentage of the bases covered at least 20%, 30%, 40%, or 50% of the average depth, was determined across four sample types. The percentage of reads on target was > 95% for all sample types.