5 PRIME Hot Master Mix

5 prime hotmaster mix logo

5 PRIME Hot Master Mix –
Highly specific Hot-Start PCR with minimal reaction setup

Optimal results – Highly specific amplification
5 PRIME Hot Master Mix is a ready-to-use reagent mix for performing hot-start PCR in a highly convenient format.  HotMaster Taq DNA polymerase, an integral component of the master mix, is designed to reduce or eliminate any non-specific products that may result from mispriming during PCR.

Minimal handling – Ready-to-use mastermix format
5 PRIME Hot Master Mix (2.5x) is a ready-to-use PCR mix that  offers high reproducibility when processing large numbers of samples.  Only primer and template need to be added to the 2.5x
concentrate, decreasing the number of time-consuming pipetting steps. This format not only reduces the likelihood of errors and the risk of contamination, but it also increases precision and sample throughput. The HotMasterMix also contains the self-adjusting Mg buffer technology . This formulation adjusts the Mg 2+ concentration automatically, eliminating the need for optimizing this critical component.

Convenience – Storage at 4°C eliminates freeze-thaw cycles
5 Prime Hot Master Mix does not need to be stored frozen, eliminating  the time-consuming thawing process and the resulting reduction in performance.
The 5 PRIME Hot Master Mix is a 2.5X concentrated with final concentrations in a 50 µl PCR reaction of 1.0 U/50 µl Taq DNA Polymerase, 45 mM KCl, 2.5 mM Mg 2+, and 200 µM of each dNTP.

5 PRIME hotmaster mix 1

Kit Content:

The 5 PRIME HotMasterMix is a 2.5x concentrated with final concentrations in a 50 µl PCR reaction of 1.0 U/50 µl Taq DNA Polymerase, 45 mM KCl, 2.5 mM Mg2+, and 200 µM of each dNTP.

Ordering:

  • Cat # 2200400: 5 PRIME Hot Master Mix – 100 rxns
  • Cat # 2200410: 5 PRIME Hot Master Mix – 1000 rxns

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