Accel-Amplicon™ BRCA1 and BRCA2 Panel

The Accel-Amplicon Plus BRCA 1 & BRCA 2 Panel is a fast, easy assay for variant discovery and screening. It offers comprehensive coverage of the entire coding sequence and flanking exon/intron boundaries of the BRCA1 and BRCA2 genes. It’s designed for compatibility with difficult and low input samples, such as FFPE and cell-free DNA for liquid biopsy analysis. But that’s just the beginning.

Assay more. Discover more.

This panel is modular allowing you to add on focused content to achieve your most optimal design. Either use our pre-validated content or add your own targets to the core. We’ll work alongside you to design your assay and then Swift will optimize and wet-bench validate its performance before shipping it to you.

Comprehensive: Covers over 1,400 COSMIC and 13,000 ClinVar mutations and generates targeted libraries compatible with Illumina® sequencing platforms.

Powerful: Simultaneous detection of single nucleotide variants (SNV), copy number variants (CNV) and small insertion and deletions (indels), all within a fast and easy single-tube assay workflow.

Flexible: Layer on pre-validated content from other Accel-Amplicon Plus Panels (TP53 and Sample_ID) or add your own targets. The possibilities are endless.

Other key features include:

  • Robust performance on cell-free DNA and FFPE samples starting with as little as 10 ng input.
  • Highly sensitive detection of rare variants down to 1% allele frequency.
  • Easy, single-tube assay with sequence ready libraries in 2 hours.
  • Streamlined analysis with bioinformatics resources such as Primerclip and Genialis.
  • Compatible with all Illumina platforms

Workflow:

The Accel-Amplicon workflow uses a fast, single-tube approach consisting of a 90-minute target enrichment amplification step and a 10-minute adapter ligation step, yielding a 2-hour start-to-finish procedure.

The single-tube workflow includes two brief incubations to generate the multiplex amplicon targets and add a unique combination of Illumina-compatible indexed adapters, creating up to 96 uniquely-indexed libraries for multiplexing on a single sequencing run.

Panel Specifications:

Performance Data:

Resources:

Protocols & Tools

Data Sheet

Ordering:

Both catalog numbers, ordered as is, provide the same panel content; however, you may add additional targets to AP-BR8048 to customize your assay. Please use Custom Target Submission Form to add additional content.

  • Cat # AP-BR8048 Accel-Amplicon™ Plus BRCA1 and BRCA2 Panel (48 rxns)
  • Cat # AL-52048 Accel-Amplicon BRCA1 and BRCA2 Panel (48 rxns)
    The below solution is included with your product. If you require extra, please advise.
  • 90196 PEG NaCl Solution (96 rxns)